Archive ouverte HAL - Diversification of the type IV filament superfamily into machines for adhesion, protein secretion, DNA uptake, and motility

Diversification of the type IV filament superfamily into machines for adhesion, protein secretion, DNA uptake, and motility

Rémi Denise 1, 2, * Sophie Abby 3 Eduardo Rocha 1
* Auteur correspondant
3 TIMC-IMAG-GEM - Génomique et Évolution des Microorganismes
TIMC-IMAG - Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525
Abstract : Processes of molecular innovation require tinkering and shifting in the function of existing genes. How this occurs in terms of molecular evolution at long evolutionary scales remains poorly understood. Here, we analyse the natural history of a vast group of membrane-associated molecular systems in Bacteria and Archaea-the type IV filament (TFF) superfamily-that diversified in systems involved in flagellar or twitching motility, adhesion, protein secretion, and DNA uptake. The phylogeny of the thousands of detected systems suggests they may have been present in the last universal common ancestor. From there, two lineages-a bacterial and an archaeal-diversified by multiple gene duplications, gene fissions and deletions, and accretion of novel components. Surprisingly, we find that the 'tight adherence' (Tad) systems originated from the interkingdom transfer from Archaea to Bacteria of a system resembling the 'EppA-dependent' (Epd) pilus and were associated with the acquisition of a secretin. The phylogeny and content of ancestral systems suggest that initial bacterial pili were engaged in cell motility and/or DNA uptake. In contrast, specialised protein secretion systems arose several times independently and much later in natural history. The functional diversification of the TFF superfamily was accompanied by genetic rearrangements with implications for genetic regulation and horizontal gene transfer: systems encoded in fewer loci were more frequently exchanged between taxa. This may have contributed to their rapid evolution and spread across Bacteria and Archaea. Hence, the evolutionary history of the superfamily reveals an impressive catalogue of molecular evolution mechanisms that resulted in remarkable functional innovation and specialisation from a relatively small set of components.
Liste complète des métadonnées

Littérature citée [93 références]  Voir  Masquer  Télécharger

https://hal-pasteur.archives-ouvertes.fr/pasteur-02263349
Contributeur : Rémi Denise <>
Soumis le : dimanche 4 août 2019 - 14:04:11
Dernière modification le : vendredi 18 octobre 2019 - 18:06:02

Fichier

journal.pbio.3000390.pdf
Publication financée par une institution

Licence


Distributed under a Creative Commons Paternité 4.0 International License

Identifiants

Collections

Citation

Rémi Denise, Sophie Abby, Eduardo Rocha. Diversification of the type IV filament superfamily into machines for adhesion, protein secretion, DNA uptake, and motility. PLoS Biology, Public Library of Science, 2019, 17 (7), pp.e3000390. ⟨10.1371/journal.pbio.3000390⟩. ⟨pasteur-02263349⟩

Partager

Métriques

Consultations de la notice

38

Téléchargements de fichiers

67