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Quantitative phase imaging of adherent mammalian cells: a comparative study

Abstract : The quantitative phase imaging methods have several advantages when it comes to monitoring cultures of adherent mammalian cells. Because of low photo-toxicity and no need for staining, we can follow cells in a minimally invasive way over a long period of time. The ability to measure the optical path difference in a quantitative manner allows the measurement of the cell dry mass, an important metric for studying the growth kinetics of mammalian cells. Here we present and compare cell measurements obtained with three different techniques: digital holographic microscopy, lens-free microscopy and quadriwave lateral sheering interferometry. We report a linear relationship between optical volume density values measured with these different techniques and estimate the precisions of this measurement for the different individual instruments.
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https://hal.archives-ouvertes.fr/hal-02181635
Contributeur : Yves Usson <>
Soumis le : dimanche 24 novembre 2019 - 18:54:05
Dernière modification le : jeudi 23 janvier 2020 - 18:22:14

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boe-10-6-2768.pdf
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C. Allier, L. Hervé, O. Mandula, P. Blandin, Y. Usson, et al.. Quantitative phase imaging of adherent mammalian cells: a comparative study. Biomedical optics express, Optical Society - SOA Publishing, 2019, 10 (6), pp.2768. ⟨10.1364/BOE.10.002768⟩. ⟨hal-02181635⟩

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