Archive ouverte HAL - A miR-150/TET3 pathway regulates the generation of mouse and human non-classical monocyte subset

A miR-150/TET3 pathway regulates the generation of mouse and human non-classical monocyte subset

Abstract : Non-classical monocyte subsets may derive from classical monocyte differentiation and the proportion of each subset is tightly controlled. Deregulation of this repartition is observed in diverse human diseases, including chronic myelomonocytic leukemia (CMML) in which non-classical monocyte numbers are significantly decreased relative to healthy controls. Here, we identify a down-regulation of hsa-miR-150 through methylation of a lineage-specific promoter in CMML monocytes. Mir150 knock-out mice demonstrate a cell-autonomous defect in non-classical monocytes. Our pulldown experiments point to Ten-Eleven-Translocation-3 (TET3) mRNA as a hsa-miR-150 target in classical human monocytes. We show that Tet3 knockout mice generate an increased number of non-classical monocytes. Our results identify the miR-150/TET3 axis as being involved in the generation of non-classical monocytes.
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https://hal.archives-ouvertes.fr/hal-02069170
Contributeur : Laurence Naiglin <>
Soumis le : vendredi 15 mars 2019 - 15:20:22
Dernière modification le : lundi 15 juillet 2019 - 15:34:02

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Dorothée Selimoglu-Buet, Julie Rivière, Hussein Ghamlouch, Laura Bencheikh, Catherine Lacout, et al.. A miR-150/TET3 pathway regulates the generation of mouse and human non-classical monocyte subset. Nature Communications, Nature Publishing Group, 2018, 9 (1), pp.5455. ⟨10.1038/s41467-018-07801-x⟩. ⟨hal-02069170⟩

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