Mechanistic Details of Early Steps in Coenzyme Q Biosynthesis Pathway in Yeast - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Cell Chemical Biology Année : 2016

Mechanistic Details of Early Steps in Coenzyme Q Biosynthesis Pathway in Yeast

Résumé

Coenzyme Q (Q) is a redox lipid that is central for the energetic metabolism of eukaryotes. The biosynthesis of Q from the aromatic precursor 4-hydroxybenzoic acid (4-HB) is understood fairly well. However, biosynthetic details of how 4-HB is produced from tyrosine remain elusive. Here, we provide key insights into this long-standing biosynthetic problem by uncovering molecular details of the first and last reactions of the pathway in the yeast Saccharomyces cerevisiae, namely the deamination of tyrosine to 4-hydroxyphenylpyruvate by Aro8 and Aro9, and the oxidation of 4-hydroxybenzaldehyde to 4-HB by Hfd1. Inactivation of the HFD1 gene in yeast resulted in Q deficiency, which was rescued by the human enzyme ALDH3A1. This suggests that a similar pathway operates in animals, including humans, and led us to propose that patients with genetically unassigned Q deficiency should be screened for mutations in aldehyde dehydrogenase genes, especially ALDH3A1.
Fichier principal
Vignette du fichier
CELL-CHEMICAL-BIOLOGY-D-16-00157_R2.pdf (1.31 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-01424510 , version 1 (05-01-2017)

Identifiants

Citer

Laurie-Anne Payet, Mélanie Leroux, John  C. Willison, Akio Kihara, Ludovic Pelosi, et al.. Mechanistic Details of Early Steps in Coenzyme Q Biosynthesis Pathway in Yeast. Cell Chemical Biology, 2016, 23 (10), pp.1241 - 1250. ⟨10.1016/j.chembiol.2016.08.008⟩. ⟨hal-01424510⟩
1927 Consultations
109 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More